ex taq dna polymerase (TaKaRa)
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ex taq dna polymerase
Ex Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 15987 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taq+polymerase/TaKaRa+Ex+Taq+DNA+Polymerase/pmc13090712-41-0-7
Average 98 stars, based on 15987 article reviews
Ex Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 15987 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taq+polymerase/TaKaRa+Ex+Taq+DNA+Polymerase/pmc13090712-41-0-7
Average 98 stars, based on 15987 article reviews
ex taq dna polymerase - by Bioz Stars,
2026-10
98/100 stars
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Polymerase Chain Reaction:Article Title: Primer Length in Reverse Transcription Determines Biodiversity Recovery and Reproducibility in
<scp>eRNA</scp>
Metabarcoding Article Snippet: .. The PCR reaction mixture was prepared in a total volume of 25 μL, consisting of 2.5 μL 10 × PCR Buffer (Takara, Japan), 2 μL dNTPs (2.5 mM each), 1.5 μL each forward and reverse primer (10 μM), 2 U Article Title: The novel (TCTG) n motif in CNBP expanded alleles: composition, dynamics and genotype-phenotype correlation in Myotonic dystrophy type 2 (DM2). Article Snippet: .. Amplification was carried out in a 30 μL volume, containing 50 ng of genomic DNA, PCR reaction buffer (5×), 25 mM MgCl2, 1.25 mM dNTPs, 70 pmol of each primer and 1U of Article Title: Comparative Enzymology and Biomass Hydrolysis Reveal Industrial Biorefining Potential of Aspergillus fumigatus Strain VP2T Article Snippet: For this purpose, Genomic DNA of the isolated fungal strain VP2T was extracted using a fungal DNA isolation kit (HiMedia; Thane, India; Cat No. MB543) and used as template to amplify approximately 600 bp of the ITS1-5.8S-ITS2 region with universal primers. .. PCR amplification was performed in a 20 μL reaction containing 1 μL DNA template, 10× Buffer, 0.2 mM dNTPs, 0.5 μM each primer, and 2 U of Article Title: Comparative Enzymology and Biomass Hydrolysis Reveal Industrial Biorefining Potential of Aspergillus fumigatus Strain VP2T. Article Snippet: For this purpose, Genomic DNA of the isolated fungal strain VP2T was extracted using a fungal DNA isolation kit (HiMedia; Thane, India; Cat No. MB543) and used as template to amplify approximately 600 bp of the ITS1-5.8S-ITS2 region with universal primers. .. PCR amplification was performed in a 20 μL reaction containing 1 μL DNA template, 10× Buffer, 0.2 mM dNTPs, 0.5 μM each primer, and 2 U of Article Title: Biosynthesis of Crystalline Selenium Nanoparticles Using Rhizospheric Bacillus cereus from Mica-Rich Agricultural Soil Article Snippet: .. The PCR amplification procedure consisted of a 25 μl mixture containing 45 ng of genomic DNA, 5 U/μl of Article Title: Aptamer-Based Discovery of Neuropilin-2 as a Potential Biomarker and Therapeutic Target in Bladder Cancer. Article Snippet: Bladder cancer (BLCA) remains a clinically challenging malignancy with high recurrence rates and limited molecular targeting options, highlighting the critical need to identify novel biomarkers and develop targeted molecular tools for improved diagnosis and therapy.. Aptamers, owing to their high affinity and specificity, represent promising molecular tools for bladder cancer detection and targeted intervention.. Using Cell-SELEX against BLCA SCaBER cells combined with counterselection against normal SV-HUC-1 cells, we successfully obtained a DNA aptamer, ss2, which was subsequently optimized to yield a truncated variant, ss2-2. Article Title: Growth Hormone Strongly Induces h SMN2 Promoter Driving Construct Gene Expression in Mammalian Cells Article Snippet: PCR amplification was performed using Thermal Cycler (Bio-Rad T100 96-Well, US) under the following conditions: initial denaturation: 94 °C 2 min (1X); first cycle: 94 °C 30s, 61 °C 30s, 72 °C 1 min (5X); following second cycle: 94 °C 30s, 65 °C 30s, 72°C 1 min. (25x); final extension: 72 °C 10 min. .. The long-range PCR for h SMN2 promoter region 3 was performed in a total volume of 50 μL and composed of 1x PCR Buffer with MgCl2, 200 μM dNTP mixture, 0.5 μM h SMN2 Forward3 primer, 0.5 μM h SMN2 Reverse primer, 500 ng Genomic DNA, 1U Amplification:Article Title: The novel (TCTG) n motif in CNBP expanded alleles: composition, dynamics and genotype-phenotype correlation in Myotonic dystrophy type 2 (DM2). Article Snippet: .. Amplification was carried out in a 30 μL volume, containing 50 ng of genomic DNA, PCR reaction buffer (5×), 25 mM MgCl2, 1.25 mM dNTPs, 70 pmol of each primer and 1U of Article Title: Comparative Enzymology and Biomass Hydrolysis Reveal Industrial Biorefining Potential of Aspergillus fumigatus Strain VP2T Article Snippet: For this purpose, Genomic DNA of the isolated fungal strain VP2T was extracted using a fungal DNA isolation kit (HiMedia; Thane, India; Cat No. MB543) and used as template to amplify approximately 600 bp of the ITS1-5.8S-ITS2 region with universal primers. .. PCR amplification was performed in a 20 μL reaction containing 1 μL DNA template, 10× Buffer, 0.2 mM dNTPs, 0.5 μM each primer, and 2 U of Article Title: Comparative Enzymology and Biomass Hydrolysis Reveal Industrial Biorefining Potential of Aspergillus fumigatus Strain VP2T. Article Snippet: For this purpose, Genomic DNA of the isolated fungal strain VP2T was extracted using a fungal DNA isolation kit (HiMedia; Thane, India; Cat No. MB543) and used as template to amplify approximately 600 bp of the ITS1-5.8S-ITS2 region with universal primers. .. PCR amplification was performed in a 20 μL reaction containing 1 μL DNA template, 10× Buffer, 0.2 mM dNTPs, 0.5 μM each primer, and 2 U of Article Title: Biosynthesis of Crystalline Selenium Nanoparticles Using Rhizospheric Bacillus cereus from Mica-Rich Agricultural Soil Article Snippet: .. The PCR amplification procedure consisted of a 25 μl mixture containing 45 ng of genomic DNA, 5 U/μl of Article Title: Aptamer-Based Discovery of Neuropilin-2 as a Potential Biomarker and Therapeutic Target in Bladder Cancer. Article Snippet: Bladder cancer (BLCA) remains a clinically challenging malignancy with high recurrence rates and limited molecular targeting options, highlighting the critical need to identify novel biomarkers and develop targeted molecular tools for improved diagnosis and therapy.. Aptamers, owing to their high affinity and specificity, represent promising molecular tools for bladder cancer detection and targeted intervention.. Using Cell-SELEX against BLCA SCaBER cells combined with counterselection against normal SV-HUC-1 cells, we successfully obtained a DNA aptamer, ss2, which was subsequently optimized to yield a truncated variant, ss2-2. Article Title: Divergent drivers of the microbial methane sink in three grassland transects and grassland types in the Northern hemisphere. Article Snippet: Understanding the multi-scale regulation of methane oxidation potential (MOP) across diverse grassland biomes is critical for predicting climate feedbacks, yet a synthetic framework across large environmental gradients remains lacking.. We combined amplicon sequencing with partial least squares path modeling to assess methane oxidation potential (MOP).. Our study spanned three Northern Hemisphere grasslands: Inner Mongolia, the Loess Plateau, and the Qinghai-Tibetan Plateau. Long Range PCR:Article Title: Growth Hormone Strongly Induces h SMN2 Promoter Driving Construct Gene Expression in Mammalian Cells Article Snippet: PCR amplification was performed using Thermal Cycler (Bio-Rad T100 96-Well, US) under the following conditions: initial denaturation: 94 °C 2 min (1X); first cycle: 94 °C 30s, 61 °C 30s, 72 °C 1 min (5X); following second cycle: 94 °C 30s, 65 °C 30s, 72°C 1 min. (25x); final extension: 72 °C 10 min. .. The long-range PCR for h SMN2 promoter region 3 was performed in a total volume of 50 μL and composed of 1x PCR Buffer with MgCl2, 200 μM dNTP mixture, 0.5 μM h SMN2 Forward3 primer, 0.5 μM h SMN2 Reverse primer, 500 ng Genomic DNA, 1U |